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bugbuster solution (Merck & Co)

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Merck & Co bugbuster solution
Bugbuster Solution, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bugbuster+solution/bugbust/pmc11735211-151-11-13
Average 90 stars, based on 1 article reviews

bugbuster solution - by Bioz Stars, 2026-10

90/100 stars

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Article Title: In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model
Article Snippet: The inclusion body fractions containing the target proteins were separated using BugBuster solution (Merck) and solubilized in buffer containing 0.3% N-lauroylsarcosine and 50 mM N-cyclohexyl-3-aminopropanesulfonicacid (CAPS; Merck; pH 11.0).

Article Title: Characterization of cysteine proteases from poultry red mite, tropical fowl mite, and northern fowl mite to assess the feasibility of developing a broadly efficacious vaccine against multiple mite species.
Article Snippet: Bacterial pellets were separated into soluble and insoluble fractions using BugBuster solution (Merck), with insoluble fractions solubilized in buffer containing 0.3% N-lauroylsarcosine and 50 mM N-cyclohexyl-3-aminopropanesulfonic acid (CAPS; Merck) (pH 11.0).

Binding Assay:

Article Title: Spectra analysis and live-neuron imaging of cyclic AMP binding domain fusing circularly permutated GFP: Data for violet light excitable cyclic AMP indicator
Article Snippet: .. Protein-expressing E. coli was collected and lysed with 3 mL BugBuster solution (Merck) for 30 min at 4 °C, followed by binding with 300 μL cobalt resin (ABT) with 30 mL buffer containing (in mM) 50 Na-phosphate, 300 NaCl, and 10 imidazole for 1 h at 4 °C. ..

Recombinant:

Article Title: Potential of histamine release factor for the utilization as a universal vaccine antigen against poultry red mites, tropical fowl mites, and northern fowl mites
Article Snippet: .. Recombinant proteins were extracted from the inclusion body fractions using the BugBuster solution (Merck). .. The proteins were solubilized in a buffer containing 0.3% N-lauroylsarcosine and 50 mM N-cyclohexyl-3-aminopropanesulfonic acid (CAPS; Merck) (pH 11.0).